超时已过期。在操作完成之前，超时时间已经过去或服务器未响应。该语句已终止。

我网站上有很多用户（每天20000-60000人次），这是一个移动文件下载站点。我可以远程访问我的服务器（Windows Server 2008-R2）。我之前收到过“服务器不可用”错误，但现在看到连接超时错误。我对此不熟悉 - 为什么会发生这种情况，如何修复它？
完整的错误信息如下：
Server Error in '/' Application. Timeout expired. The timeout period elapsed prior to completion of the operation or the server is not responding. The statement has been terminated. Description: An unhandled exception occurred during the execution of the current web request. Please review the stack trace for more information about the error and where it originated in the code.
Exception Details: System.Data.SqlClient.SqlException: Timeout expired. The timeout period elapsed prior to completion of the operation or the server is not responding. The statement has been terminated.
Source Error:
An unhandled exception was generated during the execution of the current web request. Information regarding the origin and location of the exception can be identified using the exception stack trace below. [SqlException (0x80131904): Timeout expired. The timeout period elapsed prior to completion of the operation or the server is not responding. The statement has been terminated.] System.Data.SqlClient.SqlConnection.OnError(SqlException exception, Boolean breakConnection) +404 System.Data.SqlClient.TdsParser.ThrowExceptionAndWarning() +412 System.Data.SqlClient.TdsParser.Run(RunBehavior runBehavior, SqlCommand cmdHandler, SqlDataReader dataStream, BulkCopySimpleResultSet bulkCopyHandler, TdsParserStateObject stateObj) +1363 System.Data.SqlClient.SqlCommand.FinishExecuteReader(SqlDataReader ds, RunBehavior runBehavior, String resetOptionsString) +6387741 System.Data.SqlClient.SqlCommand.RunExecuteReaderTds(CommandBehavior cmdBehavior, RunBehavior runBehavior, Boolean returnStream, Boolean async) +6389442 System.Data.SqlClient.SqlCommand.RunExecuteReader(CommandBehavior cmdBehavior, RunBehavior runBehavior, Boolean returnStream, String method, DbAsyncResult result) +538 System.Data.SqlClient.SqlCommand.InternalExecuteNonQuery(DbAsyncResult result, String methodName, Boolean sendToPipe) +689 System.Data.SqlClient.SqlCommand.ExecuteNonQuery() +327 NovinMedia.Data.DbObject.RunProcedure(String storedProcName, IDataParameter[] parameters, Int32& rowsAffected) +209 DataLayer.OnlineUsers.Update_SessionEnd_And_Online(Object Session_End, Boolean Online) +440 NiceFileExplorer.Global.Application_Start(Object sender, EventArgs e) +163 [HttpException (0x80004005): Timeout expired. The timeout period elapsed prior to completion of the operation or the server is not responding. The statement has been terminated.] System.Web.HttpApplicationFactory.EnsureAppStartCalledForIntegratedMode(HttpContext context, HttpApplication app) +4052053 System.Web.HttpApplication.RegisterEventSubscriptionsWithIIS(IntPtr appContext, HttpContext context, MethodInfo[] handlers) +191 System.Web.HttpApplication.InitSpecial(HttpApplicationState state, MethodInfo[] handlers, IntPtr appContext, HttpContext context) +352 System.Web.HttpApplicationFactory.GetSpecialApplicationInstance(IntPtr appContext, HttpContext context) +407 System.Web.Hosting.PipelineRuntime.InitializeApplication(IntPtr appContext) +375 [HttpException (0x80004005): Timeout expired. The timeout period elapsed prior to completion of the operation or the server is not responding. The statement has been terminated.] System.Web.HttpRuntime.FirstRequestInit(HttpContext context) +11686928 System.Web.HttpRuntime.EnsureFirstRequestInit(HttpContext context) +141 System.Web.HttpRuntime.ProcessRequestNotificationPrivate(IIS7WorkerRequest wr, HttpContext context) +4863749
EDIT AFTER ANSWERS: 我在Global.asax中的Application_Start如下：

protected void Application_Start(object sender, EventArgs e)
{
    Application["OnlineUsers"] = 0;

    OnlineUsers.Update_SessionEnd_And_Online(
        DateTime.Now,
        false);

    AddTask("DoStuff", 10);
}

被调用的存储过程是：

ALTER Procedure [dbo].[sp_OnlineUsers_Update_SessionEnd_And_Online]
    @Session_End datetime,
    @Online bit
As
Begin
    Update OnlineUsers
    SET
        [Session_End] = @Session_End,
        [Online] = @Online

End

我有两种获取在线用户的方法：

1. 使用 Application["OnlineUsers"] = 0;
2. 另一种方法是使用数据库

所以，对于第二种方法，我在 Application_Start 中重置了所有在线用户。该表中有超过482,751条记录。